Researchers have developed a novel test platform to assess the response to repeated stretch in the lungs during the study of breast cancer cells. The technology is designed to better understand the effects that local tissue studies on metastatic breast cancer have on how metastases proliferate in new tissue.
The study was published in the journal Advanced Functional Materials, led by a team of researchers from Purdue University.
“One of the key features of breast cancer is that most patients survive if the disease remains local, but there is a more than 70% decline in survival when cells metastasis,” Luis Solorio, an assistant professor of engineering, Said co-led the research team. “However, once the cells leave the primary tumor, they are often unresponsive to the drugs that initially worked for the patient. We wanted to develop a system that could help us better understand how new tissues in a new organ affect the invasion of tumor cells from space. “
Purdue researchers created a magnetically moving cell culture system where cancer cells can be grown in 3D on a suspended extracellular matrix protein that is abundant in early metastatic lung tissue to evaluate the effect of mechanical forces.
They were able to incorporate strain amplitude and breathing rate in this tissue mimicry. Researchers found that cells tend to divide under these conditions. The research is published in Advanced Functional Materials.
“Never before has the concept of motion been questioned as a component of the tumor microenvironment,” said Michael Wendt, a Purdue associate professor of medicinal chemistry and molecular pharmacology. “We now understand that healthy organs use momentum to resist metastatic colonialism. The development of this microactuator system will not only enhance biological understanding of metastasis, but will also serve as a platform for us to better evaluate pharmacological inhibitors of the deadliest aspect of cancer progression. “
Hyowon “Hugh” Lee, an associate professor of engineering and a researcher at the Birk Nanotechnology Center, co-led the research team.
“This is the first attempt to engineer a cell culture system that can apply mechanical forces to a suspended tissue,” Lee said. “Most bioreactors with mechanical stimulation capabilities rely on flat non-biological substrates to enhance 2D cell culture, but we are using a custom magnetic actuator and to grow 3D cells like a miniature tissue. A layer of fibronectin is suspended.
“Our system better mimics the physical environment without using artificial substrates. Using this platform, we show that some cancer cells slow down their proliferation due to cyclic stretch of breath. “
The work was a collaboration of five different laboratories to characterize the mechanical and biological properties of the new instrument.
Sarah Kellaway, a Purdue assistant professor of biomedical engineering, and Adrian Buganza Teppol, a Purdue assistant professor of mechanical engineering, interfere with the mechanical characteristics of stretching proteins. They measured the response of the material to try and develop a mapping of strains felt by cancer cells at various locations on the instrument.
Angel Enriquez, a doctoral student in Lee’s lab, said, “A key takeaway is the benefit of collaborating with people outside your area of expertise and how they can provide more thorough research.”
Sarah Liebring, a doctoral student and co-first author of the Solorio Lab, said, “It’s amazing to be part of the development of a new tool like this because by bringing together the expertise of many professors and many labs, we are now running dynamically Fibronectin is able to study cancer cells on fibrils that were not possible before. ”
(This story is published from a wire agency feed without textual modifications.)
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